Screening and Analyzing of Genes and Signaling Pathways Associated with Size Differentiation of Adult Male Prawn Macrobrachium nipponense

To better understand the size differentiation mechanism of adult male prawn Macrobrachium nipponense, transcriptome sequencing was performed from second pereiopod, hepatopancreas, and muscle M. nipponense with significant differences in (large: 1.47 ± 0.12 g; small: 0.28 0.04 g). A total 46,557 unigenes an average length 1,312 bp were obtained. Based on pereiopod large (LP) versus small (SP), (LM) (SM), hepatopancreas (LH) (SH), a 805, 473, 1,037 significantly upregulated DEGs identified, respectively. The KEGG pathway analysis indicated that pathways such as PI3K-Akt pathway, cAMP Hippo Ras signaling enriched all three comparisons, which may be key regulators nipponense. Furthermore, potential candidates for prawns, twenty-eight involved these four screened out, their expressions verified by quantitative real-time PCR (qPCR). results this study preliminarily elucidate molecular provide theoretical reference improving commercial cultured