<scp>miR</scp>‐643 suppresses cell invasion and radioresistant of lung cancer through <scp>RAF1</scp>

RAF1 (c-raf) has been known as an important tumor-promoter in many cancers. However, the regulatory mechanisms affecting expressions are rarely reported. This study aimed to predict candidate miRNAs of gene and verify their functions progression lung cancer. The expression miR-643 was detected by reverse transcription polymerase chain reaction (RT-PCR). A dual-luciferase report system used relationship between RAF1. RT-PCR Western blot were analyze Transwell chamber, scratch, monoclonal tests showed that affected proliferation, migration, radiosensitivity H1299 A549 cells targeting gene. cancer lower than bronchial epithelioid (CRL-2741), luciferase reporter experiment confirmed targeted MiR-643 overexpression decreased expression, thereby decreasing cell radiation resistance through AKT/nuclear factor-κB pathway. could inhibit invasion, metastasis increase downregulating