Schisandrin B Inhibits Osteosarcoma Cell Proliferation and Promotes Apoptosis Through PI3K/AKT/mTOR Pathway Mediated Autophagy

Abstract Osteosarcoma is the most common primary malignant bone tumor; main treatment method surgery and adjuvant chemotherapy, with a 5-year survival rate of less than 20% for metastatic patients. Schisandrin B abundant active ingredient found in fruit Schisandra chinensis (Turcz.) Baill., Schisandraceae, which has document properties such as liver protection, antioxidant, anti-inflammatory, antiaging, antitumor. The present investigation explored therapeutic effect schisandrin on osteosarcoma (MG63 cells). Cell proliferation viability, scratch assay, transwell migration analysis were used to detect effects growth activity, migration, invasion MG63 cells. cell apoptosis detected by flow cytometry tunel staining. Western blot was expression levels autophagy related proteins. Immunofluorescence staining inhibited ability, ability In addition, induced cells through mediated PI3K/AKT/mTOR signaling pathway. Graphical