SCF-Fbxo42 promotes synaptonemal complex assembly by downregulating PP2A-B56

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PP2A-B56 opposes Mps1 phosphorylation of Knl1 and thereby promotes spindle assembly checkpoint silencing

The spindle assembly checkpoint (SAC) monitors correct attachment of chromosomes to microtubules, an important safeguard mechanism ensuring faithful chromosome segregation in eukaryotic cells. How the SAC signal is turned off once all the chromosomes have successfully attached to the spindle remains an unresolved question. Mps1 phosphorylation of Knl1 results in recruitment of the SAC proteins ...

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PP2A-B56 opposes Mps1 phosphorylation of Knl1

Mps1-dependent spindle assembly checkpoint (SAC) signaling delays anaphase entry until all chromosomes have been correctly attached to the mitotic spindle (Foley and Kapoor, 2013). Knl1 is the kinetochore localized binding partner for the SAC proteins Bub1, BubR1, and Bub3 (Kiyomitsu et al., 2007). Phosphorylation of Knl1 by Mps1 is a prerequisite for the interaction of Bub1 and Bub3 with Knl1 ...

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Proper alignment of duplicated chromosomes at the metaphase plate involves both motor-driven chromosome movement and the functional and physical end-on connection (K-fiber formation) between the kinetochore and the plus-end of microtubules. The B56 family of protein phosphatase 2A (PP2A) regulatory subunits (B56-PP2A), through their interaction with the mitotic checkpoint protein BUBR1, are req...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 2020

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.202009167