Scaling Up DNA Computing with Array-Based Synthesis and High-Throughput Sequencing

Abstract It was 40 years ago today, when Ned taught DNA to play [32]. When Seeman began laying the theoretical foundations of what is now nanotechnology, he likely did not imagine entire diversity and scale molecular structures, machines, computing devices that would be enabled by his work. While there are many reasons for success field, least creativity shown community helped build, such progress have been possible without breakthroughs in synthesis analysis technology. Here, we argue technologies will enable next generation nanotechnology already arrived but yet fully taken advantage them. Specifically, believe it become possible, near future, dramatically up through use array-synthesized high-throughput sequencing. In this article, provide an example how logic gates circuits can produced enzymatic processing read out sequencing a massively parallel format. We experimentally demonstrate readout 380 single reaction. further speculate longer term, very large-scale find applications context diagnostics and, particular, data storage.