SARS-CoV-2 Neutralizing Antibody Responses towards Full-Length Spike Protein and the Receptor-Binding Domain

Abstract Tools to monitor SARS-CoV-2 transmission and immune responses are needed. We present a neutralization ELISA determine the levels of Ab-mediated virus preclinical model focused immunization strategy. The is strongly correlated with elaborate plaque reduction test (? = 0.9231, p < 0.0001). potency convalescent sera correlates IgG titers against receptor-binding domain (RBD) spike 0.8291 0.8297, respectively; 0.0001) lesser extent protein N 0.6471, vaccine NMRI mice models using RBD full-length Ag as immunogens show profound Ab capacity (IC50 1.9 × 104 2.6 3.9 103 5.2 103, respectively). Using panel novel high-affinity murine mAbs, we also that majority RBD-raised mAbs have inhibitory properties, whereas only few spike-raised do. ELISA-based viral offers time- cost-effective alternative test. results indicate strategies on region may advantages compared full spike.