Şap Hastalığı Teşhisi için Enzim Immunotransfer Blot Testinin kantitatif bir yaklaşım ile iyileştirilmesi

Foot-and-mouth disease (FMD) is a highly contagious animal that causes devastating economic losses. The trade of live animals and derived products only possible if the exporting country free from disease, according to World Organization for Animal Health (WOAH) code FMD. One most important ways prove disease-free measure levels non-structural protein antibodies (NPS) FMD virus in target population sampled. For detection status herd, mass screening assays such as Enzyme-linked immunosorbent assay (ELISA) immunoelectrotransfer blot (EITB) were developed described WOAH diagnostic manual. In this study, recombinant FMDV NS proteins produced tested with sera panels collected uninfected naturally infected using quantitative Western an improved EITB, which enables numerical documentation statistical analysis. NSP band intensities used determine cut-off values, differentiating non-infected revealing variable sensitivity among different NSPs. EITB results also showed correlation NSP-ELISA results.