منابع مشابه
Sandwich enzyme immunoassay of osteocalcin in serum with use of an antibody against human osteocalcin.
We have developed and thoroughly validated a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) on microtiter plates for osteocalcin in human serum with use of an antibody raised against human osteocalcin. We used a monoclonal antibody against bovine osteocalcin as the capture antibody; the second antibody was a polyclonal antibody against human osteocalcin. The amount of bound seco...
متن کاملOptimization of the cutoff value for the Aspergillus double-sandwich enzyme immunoassay.
BACKGROUND Many health care centers worldwide use the Platelia Aspergillus enzyme immunoassay (PA-EIA; Bio-Rad Laboratories) for diagnosis of invasive aspergillosis (IA). A cutoff optical density (OD) index of 1.5 was originally recommended by the manufacturer, but in practice, most institutions use lower cutoff values. Moreover, a cutoff OD index of 0.5 was recently approved in the United Stat...
متن کاملA new sandwich enzyme immunoassay for measurement of plasma pre-beta1-HDL levels.
Pre-beta1-HDL, a putative discoid-shaped high density lipoprotein (HDL) of approximately 67-kDa mass that migrates with pre-beta mobility in agarose gel electrophoresis, contains apolipoprotein A-I (apoA-I), phospholipids, and unesterified cholesterol. It participates in the retrieval of cholesterol from peripheral tissues. In this study we established a new sandwich enzyme immunoassay (EIA) fo...
متن کاملClinical implications of a sandwich enzyme immunoassay for big endothelin-1.
Endothelin (ET) is an endothelial-derived peptide that exerts potent vasoconstrictive activity in vivo and in vitro. An increasing body of evidence suggests that ET may have a significant role in the pathophysiological processes leading to cardiovascular disease states. Although it is not certain at this point whether ET is primarily responsible for initiating these events, sufficient evidence ...
متن کاملSandwich enzyme immunoassay of cystatin C in serum with commercially available antibodies.
We developed a sandwich enzyme immunoassay for determining cystatin C in serum by using commercially available antibodies. We optimized each assay step (e.g., concentrations of coating rabbit anti-human cystatin C antibodies and horseradish peroxidase-conjugated antibodies) and studied the binding kinetics of antigen and antibodies. The within-assay CV was < 5%, the between-assay CV was 8.8%, t...
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ژورنال
عنوان ژورنال: Journal of Pharmacobio-Dynamics
سال: 1982
ISSN: 0386-846X,1881-1353
DOI: 10.1248/bpb1978.5.708