Saliva from nymph and adult females of Haemaphysalis longicornis: a proteomic study
نویسندگان
چکیده
منابع مشابه
Proteomic Screening of Antigenic Proteins from the Hard Tick, Haemaphysalis longicornis (Acari: Ixodidae)
Proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. For detection of antigens from Haemaphysalis longicornis, 1-dimensional electrophoresis (1-DE) quantitative immunoblotting technique combined with 2-dimensional electrophoresis (2-DE) immunoblotting was used for whole body proteins from unfed and partially fed...
متن کاملDrug Induced Sialorrhea and Microfluidic-Chip-Electrophoretic Analysis of Engorged Adult Female Tick Saliva of Haemaphysalis longicornis (Acari: Ixodidae)
BACKGROUND The aim of the present study was to induce salivation in Haemaphysalis longicornis to increase saliva production and to characterize the collection of proteins present in the collected saliva using on-chip-electrophoresis. METHODS Saliva of adult female engorged H. longicornis was collected by treatment with 0.2% dopamine hydrochloride. All protein samples were characterized by SDS...
متن کاملdrug induced sialorrhea and microfluidic-chip-electrophoretic analysis of engorged adult female tick saliva of haemaphysalis longicornis (acari: ixodidae)
background: the aim of the present study was to induce salivation in haemaphysalis longicornis to increase saliva production and to characterize the collection of proteins present in the collected saliva using on-chip-electrophoresis. methods: saliva of adult female engorged h. longicornis was collected by treatment with 0.2% dopamine hydrochloride. all protein samples were characterized by sd...
متن کاملa study on the design of bio-ethanol process from date wastes of sistan and baluchistan province
اتانول کاربردهای متنوعی در صنایع لاستیک سازی، رنگسازی، حلالها ومکمل سوخت خودرو دارد. اتانول برخلاف نفت از جمله مواد تجدیدپذیر محسوب می شود که مشکلات زیست محیطی و آلودگی نیز ایجاد نمی کند. استفاده از اتانول به عنوان مکمل سوختخودروها از جمله مهمترین مصارف صنعتی این ماده بشمار می رود. با توجه به این موضوع تحقیق و توسعه در زمینه تولید اتانول با درجه خلوص بالا در سطح جهان، و نه تنها در کشور های پیشر...
Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis
Vaccination is considered a promising alternative for controlling tick infestations. Haemaphysalis longicornis midgut proteins separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane were screened for protective value against bites. The western blot demonstrated the immunogenicity of 92 kDa protein (P92). The analysis of the P92 amino acid sequence by LC-MS/MS indicat...
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ژورنال
عنوان ژورنال: Parasites & Vectors
سال: 2015
ISSN: 1756-3305
DOI: 10.1186/s13071-015-0918-y