SAFETY EVALUATION OF DE-020 EYE DROPS : Eye irritation test and subacute toxicity test in rabbits

Draize Eye Irritancy Test Rabbits

Household products, shampoos, pesticides, weed killers, even riot control gases, are just some of the products tested for irritancy in the eyes of conscious rabbits. The Draize test, introduced in 1944, is cruelly simple. The substance under test is sprayed or instilled into one eye of an albino rabbit. Generally no pain relief is given and the test often proceeds for 7 days during which the co...

Provocative test combining water drinking and homatropine eye drops

In a previous study (Leighton, Phillips, and Gibbs, 1970), a provocative outflow test using a combination of water drinking (20 ml./kg. body weight) and gutt. homatropine 2 per cent. in 24 open-angle glaucoma suspects caused a significantly greater fall in outflow facility and rise in outflow resistance than either water drinking alone or homatropine alone (0o02 < P < o0os in each case). When a...

The Isolated Chicken Eye test to replace the Draize test in rabbits.

In 1944, Draize et al., published a paper entitled "Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes". The Organization for Economic Co-operation and Development published their first guideline on eye irritation in 1981, using rabbits. In the early eighties the development of alternative non-animal tests to replace the Draize eye ...

Efficacy of retinol palmitate eye drops for dry eye in rabbits with lacrimal gland resection

PURPOSE We examined the efficacy of retinol palmitate (VApal) for dry eyes using dry eye model rabbits whose lacrimal glands were resected. MATERIALS AND METHODS After alkaline injury on keratoconjunctival epithelium, VApal eye drops were administered 6 times a day for 7 days. The efficacy of VApal was also compared with that of 0.1% hyaluronic acid eye drops. RESULTS The fluorescein staini...

Application of Alternative Methods in the Regulatory Assessment of Chemical Safety Related to Human Eye Irritation & Severe Eye Irritation