Rubisco Small Subunits’ Genome-Wide Identification and Their Function from Gene Expression to Rubisco Activity and Photosynthesis among Peanut Genotypes under Different Nitrogen Levels

Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a critical protein for photosynthesis. With small subunits, RBCSs catalyze the fixation of CO2 onto ribulose-1,5-bisphosphate (RuBP). However, gene characteristics peanut RBCSs, and relationship between their expression growth under nitrogen (N)-deficiency conditions are not yet known. This study targeted impact sufficient N (5 mmol/L NO3?) deficient (0.1 on seedlings’ growth, physiology, molecular aspects. Herein, eight RBCS encoding genes were identified in genome, described. The leaf areas Yu Hang Hua 1 (YH1) An 3 (AH3) reduced significantly after 10 days while “Pu Ke 18” (PK18) “Luo 4087” (LH4087) had higher N-deficiency adaptations initial stage. Rubisco carboxylation activities YH1 PK18 increased 5 stress they decreased 15 conditions. Furthermore, qRT-PCR results showed that expressions AhRBCS 1/5 AhRBCS2/6 negative relationships with morpho-physiological traits low-N stress. provides biological information lays theoretical foundation utilization crop plants.