Rotational Diffusion of Eosin-Labeled Pyruvate Dehydrogenase Complex of Escherichia coli
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چکیده
منابع مشابه
Reconstitution of the Escherichia coli pyruvate dehydrogenase complex.
The binding of pyruvate dehydrogenase and dihydrolipoyl dehydrogenase (flavoprotein) to dihydrolipoyl transacetylase, the core enzyme of the E. coli pyruvate dehydrogenase complex [EC 1.2.4.1:pyruvate:lipoate oxidoreductase (decaryboxylating and acceptor-acetylating)], has been studied using sedimentation equilibrium analysis and radioactive enzymes in conjunction with gel filtration chromatogr...
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The investigation of the substrate specificity of the pyruvate dehydrogenase complex from Escherichia coli allows a description of the binding region of pyruvate. Substrate analogs with electronegative substitutions in the methyl group show a strong competitive inhibition of the overall reaction of the pyruvate dehydrogenase complex. The most efficient inhibitor is fluoropyruvate which has a mo...
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This postulated sequence is based mainly on experimental observations concerning the cofactor requirements for the overall reaction (2-5)) exchange reactions (6, 7)) and model reactions involving the enzymatic interaction of lipoic acid with acetyl CoA (8, 9) and reduced lipoic acid with DPN (10). It has been presumed that each of the four reactions is catalyzed by a separate enzyme, although a...
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Danson, M. J. & Perham, R. N. (1976) Biochem. J. 159, 677-682 Danson, M. J., Hooper, E. A. & Perham, R. N. (1978a) Biochem. J. 175, 193-198 Danson, M. J., Fersht, A. R. & Perham, R. N. (1978b) Proc. Natl. Acad. Sci. U.S.A. in the press Durchschlag, H. (1975) Biophys. Struct. Mech. 1 169-188 Eley, M. H., Namihara, G., Hamilton, L., Munk, P. & Reed, L. J. (1972) Arch. Biochem. Biophys. 152, 655-6...
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In addition to the similarities in enzymic activities, UvrD protein also possesses similar chromatographic properties to helicase 11, eluting from DNA-agarose at 0.4M-~dt, phospho-cellulose at 0.35~ and DEAEcellulose at 0 . 1 8 ~ , which are all close to previously reported values for helicase 11. Furthermore, both proteins behave as monomers in gel filtration, eluting just before bovine serum ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1981
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1981.tb06453.x