Roles of outer membrane protein W (OmpW) on survival, morphology, and biofilm formation under NaCl stresses in Cronobacter sakazakii
نویسندگان
چکیده
منابع مشابه
Genes involved in Cronobacter sakazakii biofilm formation 1 Running title : Analysis of biofilm formation of Cronobacter sakazakii 2 3 Isabel
2 Department of Microbiology, Institute of Plant Biology, University of Zürich, 9 Winterthurerstasse 190, 8057 Zürich, Switzerland 10 11 12 13 14 *Corresponding author. Mailing address: Institute for Food Safety and Hygiene, 15 Winterthurerestrasse 272, 8057 Zürich, Switzerland; Phone: +41 44 635 86 51; Fax: +41 44 635 16 89 08 ; E-Mail: [email protected] 17 18 19
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The outer-membrane protein OmpW of Vibrio cholerae was studied with respect to its structure, functional properties and regulation of expression. On SDS-PAGE, the membrane-associated form of OmpW protein (solubilized by either 0.1 % or 2 % SDS at 25 degrees C) migrated as a monomer of 19 kDa that changed to 21 kDa on boiling. The protein was hyperexpressed in Escherichia coli in the histidine-t...
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Cronobacter species are foodborne pathogens that can cause neonatal meningitis, necrotizing enterocolitis, and sepsis; they have unusual abilities to survive in environmental stresses such as acid stress. However, the factors involved in acid stress responses and biofilm formation in Cronobacter species are poorly understood. In this study, we investigated the role of grxB on cellular morpholog...
متن کاملThymoquinone Inhibits Virulence Related Traits of Cronobacter sakazakii ATCC 29544 and Has Anti-biofilm Formation Potential
The aim of this study was to determine whether thymoquinone, the principal active ingredient in the volatile oil of Nigella sativa seeds, could suppress certain virulence traits of Cronobacter sakazakii ATCC 29544 which contribute to infection. Sub-inhibitory concentrations of thymoquinone significantly decreased motility, quorum sensing, and endotoxin production of C. sakazakii ATCC 29544 and ...
متن کاملSurvival and growth of Cronobacter species (Enterobacter sakazakii) in wheat-based infant follow-on formulas.
AIMS To determine the survival and growth characteristics of Cronobacter species (Enterobacter sakazakii) in infant wheat-based formulas reconstituted with water, milk, grape juice or apple juice during storage. METHODS AND RESULTS Infant wheat-based formulas were reconstituted with water, ultra high temperature milk, pasteurized grape or apple juices. The reconstituted formulas were inoculat...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 2018
ISSN: 0022-0302
DOI: 10.3168/jds.2017-13791