Roles of LuxR in regulating extracellular alkaline serine protease A, extracellular polysaccharide and mobility ofVibrio alginolyticus
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منابع مشابه
Role of alkaline serine protease, asp, in vibrio alginolyticus virulence and regulation of its expression by luxO-luxR regulatory system.
The alkaline serine protease asp, which was shown to be a virulence factor of Vibrio alginolyticus as a purified protein, was cloned from V. alginolyticus EPGS, a strain recently isolated from moribund Epinephelus coioides in an outbreak of vibriosis in a mariculture farm of Shenzhen. The asp null mutant was constructed by homologous recombination with suicide plasmid pNQ705-1. Compared with th...
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in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...
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Production of complex extracellular polysaccharides (EPSs) by the nitrogen-fixing soil bacterium Sinorhizobium meliloti is required for efficient invasion of root nodules on the host plant alfalfa. Any one of three S. meliloti polysaccharides, succinoglycan, EPS II, or K antigen, can mediate infection thread initiation and extension (root nodule invasion) on alfalfa. Of these three polysacchari...
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Proteases are very important industrial enzymes, which contribute about 60% of the total world enzyme market. Among the various proteases, bacterial proteases are the most significant, compared with animal and fungal proteases (Rahman et al., 1994) and among bacteria, Bacillus sp are specific producers of extra-cellular proteases (Coolbear et al., 1992; Banerjee et al., 1999). These enzymes hav...
متن کاملCharacterization of extracellular alkaline proteases and collagenase induction in Vibrio alginolyticus.
The number and approximate molecular weights of extracellular alkaline proteases produced by Vibrio alginolyticus were determined by gelatin-PAGE. Three major bands of protease activity with apparent molecular weights of approximately 28 000, 22 500 and 19 500 (proteases 1, 2 and 3, respectively) and two minor bands of protease activity with apparent molecular weights of approximately 15 500 an...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2008
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2008.01185.x