Roles of alpha-toxin and beta-toxin in virulence of Staphylococcus aureus for the mouse mammary gland

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Roles of alpha-toxin and beta-toxin in virulence of Staphylococcus aureus for the mouse mammary gland.

Mutants of Staphylococcus aureus which fail to express alpha-toxin (Hly), beta-toxin (Hlb), or both have been constructed by site-specific mutagenesis. The virulence of the mutants was compared with that of wild-type toxigenic strains by intramammary inoculation of lactating mice. A bovine strain, M60, and a laboratory strain, 8325-4, caused acute mastitis and death within 48 h for 60% of the m...

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Septic arthritis is a common and feared complication of staphylococcal infections. Staphylococcus aureus produces a number of potential virulence factors including certain adhesins and enterotoxins. In this study we have assessed the roles of cytolytic toxins in the development of septic arthritis by inoculating mice with S. aureus wild-type strain 8325-4 or isogenic mutants differing in the ex...

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The structure of Staphylococcus aureus alpha-toxin-induced ionic channel.

Polyethylene glycols (PEG) with molecular weight less than or equal to 3000 were shown to effectively protect human erythrocytes from osmotic lysis induced by alpha-staphylotoxin (ST). PEG with MW less than 3000 do not change the conductivity of ion channels induced by ST in bilayer lipid membranes (BLM). Changing the bilayer from a pure phosphatidylcholine (PC) to a negatively charged phosphat...

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Diminished virulence of an alpha-toxin mutant of Staphylococcus aureus in experimental brain abscesses.

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 1989

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.57.8.2489-2494.1989