RNAi-mediated DNA silencing
نویسندگان
چکیده
منابع مشابه
RNAi-mediated DNA silencing
Tucker, and Edwin Chapman (University of Wisconsin, Madison, WI) find that PIP 2 is a plasma membrane dock for synaptotagmin-1 (syt), a transmembrane protein localized in secretory vesicle membranes, when calcium is absent. This dock may ensure speedy and directed fusion in response to calcium influx. The syt dock has two calcium-binding domains in its cytoplasmic region, called C 2 A and C 2 B...
متن کاملRNAi-mediated Gene Silencing and Its Implications for Agriculture
Background RNA interference (RNAi) has emerged as a powerful genetic tool for scientific research over the past several years. RNAi is known as a conserved integral part of the gene regulation processes present in all eukaryotes that utilizes small RNAs (sRNAs) to direct the silencing of gene expression at i.a. the post-transcriptional level. Posttranscriptional gene silencing (PTGS) starts wit...
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Crown gall disease, caused by the soil bacterium Agrobacterium tumefaciens, results in significant economic losses in perennial crops worldwide. A. tumefaciens is one of the few organisms with a well characterized horizontal gene transfer system, possessing a suite of oncogenes that, when integrated into the plant genome, orchestrate de novo auxin and cytokinin biosynthesis to generate tumors. ...
متن کاملRNAi-Mediated Gene silencing in Zebrafish Triggered by Convergent Transcription
RNAi based strategies to induce gene silencing are commonly employed in numerous model organisms but have not been extensively used in zebrafish. We found that introduction of transgenes containing convergent transcription units in zebrafish embryos induced stable transcriptional gene silencing (TGS) in cis and trans for reporter (mCherry) and endogenous (One-Eyed Pinhead (OEP) and miR-27a/b) g...
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I n 1925, Gershenson started laboratory cultures from 19 female Drosophila obscura that were collected from a forest near Moscow. After recounting his difficulties raising the flies (partial success achieved with a diet of potatoes and fermented raisins), he noted that progeny from most cultures contained an approximately equal sex ratio [1]. Several cultures, however, yielded progeny with high...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2004
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb1643rr5