RNAi Degrades Spike Protein Gene Transcripts of the SARS-CoV-2 for Developing Drugs to Treat of COVID-19

COVID-19 is caused by severe acute respiratory SARS-CoV-2. Regardless of the availability treatment strategies for COVID-19, effective therapy will remain essential. A promising approach to tackle SARS-CoV-2 could be small interfering (si) RNAs. Here we designed hairpin RNA (named as shRNA688) targeting prepared 813 bp Est S protein genes (Delta). The conserved and mutated regions from genomes variants in public database were analyzed. fragment encoding most part RBD partial downstream was cloned into upstream red florescent gene (RFP) a fusing pCMV-S-Protein RBD-Est-RFP plasmid expressing potential target RNAi. double stranded DNA shRNA688 constructed human H1 promoter which CMV drives enhanced green fluorescent (EGFP) marker expression. These two kinds plasmids co-transfected HEK293T via Lipofectamine 2000. degradation transcripts expressed transfected treated RNAi analyzed RT-qPCR with specific probe targeted transcripts. Our results showed that region effectively reduce genes. This study provides cell model technical support research development broad-spectrum nucleic acid drugs against or other hazard viruses cause diseases.