RNA isolation using immobilized PNA

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

Expanding the repertoire of pyrrolidyl PNA analogues for DNA/RNA hybridization selectivity: aminoethylpyrrolidinone PNA (aepone-PNA).

New PNA analogues derived from aminoethylpyrrolidin-5-one backbone show stabilization of aepone-PNA:DNA hybrids and destabilization of the corresponding RNA hybrids compared to unmodified PNA.

RNA Interference in Mammalia Cells by RNA-3’-PNA Chimeras

The discovery of siRNAs as the mediators of RNA interference has led to an increasing interest in their therapeutic applications. Chemical modifications are introduced into siRNAs to optimize the potency, the stability and the pharmacokinetic properties in vivo. Here, we synthesize and test the effects of RNA-3'-PNA chimeras on siRNA functioning and stability. We demonstrate that the chemical m...

face-Immobilized Aptamers for Cancer Cell Isolation

Downloa osing rare but highly malignant tumor cells that migrate from the primary tumor mass into adjacent (s) or circulate in the bloodstream is critical for early detection and effective intervention(s). Here, ort on an aptamer-based strategy directed against epidermal growth factor receptor (EGFR), the most on oncogene in glioblastoma (GBM), to detect these deadly tumor cells. GBMs are chara...

RNA isolation (Total RNA Extraction from High Polysaccharide Plants). Fatemeh Mehrpooyan*