RNA editing by T7 RNA polymerase bypasses InDel mutations causing unexpected phenotypic changes
نویسندگان
چکیده
منابع مشابه
RNA editing by T7 RNA polymerase bypasses InDel mutations causing unexpected phenotypic changes
DNA-dependent T7 RNA polymerase (T7 RNAP) is the most powerful tool for both gene expression and in vitro transcription. By using a Next Generation Sequencing (NGS) approach we have analyzed the polymorphism of a T7 RNAP-generated mRNA pool of the mboIIM2 gene. We find that the enzyme displays a relatively high level of template-dependent transcriptional infidelity. The nucleotide misincorporat...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2015
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkv269