Ribosomal Binding Site Switching: An Effective Strategy for High-Throughput Cloning Constructions
نویسندگان
چکیده
منابع مشابه
Ribosomal Binding Site Switching: An Effective Strategy for High-Throughput Cloning Constructions
Direct cloning of PCR fragments by TA cloning or blunt end ligation are two simple methods which would greatly benefit high-throughput (HTP) cloning constructions if the efficiency can be improved. In this study, we have developed a ribosomal binding site (RBS) switching strategy for direct cloning of PCR fragments. RBS is an A/G rich region upstream of the translational start codon and is esse...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2012
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0050142