Ribonucleic acid synthesis in isolated rat liver nuclei under conditions of ribonucleic acid processing and transport
نویسندگان
چکیده
منابع مشابه
Ribonucleic Acid Composition of Rat Liver Tumor
Studies on normal tissues have suggested that the ribonucleic acid (RNA) of nuclei, mitochon dna, microsomes, and supernatant fluid isolated from tissue homogenate has a characteristic nu cleotide composition (7, 8, 16), while other data did not support such a view (6). Results obtained in this laboratory revealed, in fact, the occurrence of characteristic ribonucleic acids in rat liver frac ti...
متن کاملIsolation and characterization of uridylic acid-rich 7 S ribonucleic acid of rat liver nuclei.
Morphologically intact nuclei were prepared from 1 to 1.5 kg of rat liver by batch techniques with the use of a modified Hobart tissue grinder, continuous homogenizers, and discontinuous sucrose gradients in Sharples centrifuges. Undegraded nuclear RNA was recovered in yields averaging 73 mg per kg of rat liver. For isolation of the 4 to 7 S RNA, whole nuclear RNA was loaded in a zonal rotor in...
متن کاملLocalization of deoxyribonucleic acid complementary to ribosomal ribonucleic acid and preribosomal ribonucleic acid in the nucleolus of rat liver.
The fraction and distribution of DNA complementary to ribosomal RNA and preribosomal RNA was investigated by the technique of molecular hybridization with the DNA of isolated nucleoli and nuclei of rat liver. Nuclear DNA complementary to ribosomal 18 S RNA and 28 S RNA was 0.014% and 0.032%, respectively. Nucleolar DNA complementary to ribosomal 18 S RNA and 28 S RNA was 0.10% and 0.22%, respec...
متن کاملEnzymatic Synthesis of Ribonucleic Acid
The discovery of enzymes which catalyze the deoxyribonucleic acid-dependent synthesis of ribopolynucleotides (l-6) stimulated an immediate inquiry into the role played by DNA in thisunique reaction. The speculation that DNA might serve as a template for the polymerization of ribonucleotides into a complementary ribonucleic acid molecule was confirmed by studies from a number of different groups...
متن کاملThe site of ribonucleic acid synthesis in the isolated nucleus.
There is still controversy concerning the primary site of RNA synthesis in the cell.' When tissues are radioautographed after administration of radioactive inorganic phosphorus or radioactive RNA2 precursors such as tritiated uridine or cytidine, it is commonly observed that radioactivity appears in the nucleus first and in the cytoplasm later. It has furthermore been shown that isolated nuclei...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1975
ISSN: 0264-6021
DOI: 10.1042/bj1470193