Ribonucleic Acid Polymerase from Micrococcus luteus
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منابع مشابه
Ribonucleic Acid Polymerase from Micrococcus Zysodeikticus
The homopolymer complex, (dA, .dT,), will direct the synthesis of r(A),, or r(U),,, or both, in the presence of highly purified RNA polymerase from iVIicrococcus Zysodeikticus. When either ‘GATP or 3H-UTP is present with saturating amounts of (dA, . dT,), AMP incorporation is considerably greater than UMP incorporation. This difference appears to reflect a product inhibition which is observed o...
متن کاملEnzymatic Synthesis of Ribonucleic Acid. I. Preparation of Ribonucleic Acid Polymerase from Extracts of Micrococcus Lysodeikticus.
The problem of complex polynucleotide synthesis has received considerable attention from numerous investigators (l-3). Grunberg-Manago and Ochoa (4) and Kornberg, Lehman, and Simms (5) first successfully demonstrated the polymerization in vitro of riboand deoxynucleotides with cell-free extracts from microorganisms. Subsequent studies on the synthesis of ribonucleic acid and deoxyribonucleic ac...
متن کاملEnzymatic Synthesis of Ribonucleic Acid 1. PREPARATION OF RIBONUCLEIC ACID POLYMERASE FROM EXTRACTS OF MICROCOCCUS LYSODEIKTICUP
The problem of complex polynucleotide synthesis has received considerable attention from numerous investigators (l-3). Grunberg-Manago and Ochoa (4) and Kornberg, Lehman, and Simms (5) first successfully demonstrated the polymerization in vitro of riboand deoxynucleotides with cell-free extracts from microorganisms. Subsequent studies on the synthesis of ribonucleic acid and deoxyribonucleic ac...
متن کاملThe incorporation of sangivamycin 5'-triphosphate into polyribonucleotide by ribonucleic acid polymerase from Micrococcus lysodeikticus.
Sangivamycin is incorporated into RNA in the reaction catalyzed by the RNA polymerase from Micrococcus lysodeikticus with the use of calf thymus DNA or the copolymer of deoxyadenosine and deoxythymidine (poly d(A-T)). The incorporation of sangivamycin 5’4riphosphate into RNA is dependent on primer, time, protein concentration, and Mn++ or Mg+f. When calf thymus DNA is primer, GTP, CTP, and UTP ...
متن کاملEnzymatic Synthesis of Ribonucleic Acid. Ii. Properties of the Deoxyribonucleic Acid-primed Reaction with Micrococcus Lysodeikticus Ribonucleic Acid Polymerase.
The discovery of enzymes which catalyze the deoxyribonucleic acid-dependent synthesis of ribopolynucleotides (l-6) stimulated an immediate inquiry into the role played by DNA in thisunique reaction. The speculation that DNA might serve as a template for the polymerization of ribonucleotides into a complementary ribonucleic acid molecule was confirmed by studies from a number of different groups...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63532-x