RFLP in human F13A gene

MspI RFLP in the human fumarylacetoacetate hydrolase (FAH) gene.

A TaqI RFLP for the human arylsulfatase A gene.

A frequent Hindlll RFLP of the human fibrorrertin gene ( FNl )

SOURCE/DESCRIPTION: The probe used is a Hindlll/BamHI fragment of 1.4kb from the fibronectin recombinant, pFH6 (Kornblihtt, A.R. et al.). POLYMORPHISM: Hindlll (A/AGCTT) (New England, Bio-Labs, FRG) detects invariant bands at 4.75 and 1.7kb in addition to a simple two-allele polymorphism defined by the presence of a band of 7.1kb (F2) alternatively with two bands of 3.7 and 3.4kb (Fl) (Figure 1...

StuI RFLP in the human ABL locus.

Source/Description: A 3.6 kb EcoRI fragment (lambda ABL3) containing portions of exons 7 and 8 and the intervening intron of the ABL oncogene was isolated from a chromosome 9-specific library (ATCC # LA09NS01) using a 1.7 kb Abelson murine leukemia virus probe (v-abl, ONCOR, Inc.). A single copy 1.8 kb BglII fragment (ABL3) was derived from the original lambda clone and detects the StuI RFLP. P...

PvuII RFLP in the cytovillin gene (VIL2) on human chromosome 6q.

Figure 1. IFNGR1 RFLP detected by Southern blot analysis of Taql digested human DNA. The two alleles arc identified by 6. lkb (Al) and 3.2+2.9kb (A2) bands in a .8% agarosegel. Lane 1: Al homozygous; lane 2: A2 homozygous; lane 3: heterozygous. Hybridization and washing conditions were 50% formamide, 3x SSC, 37°C and .2x SSC, 60°C, respectively. figure l.VIL 2 RFLP detected by Southern Blot ana...