Review: Immunologic Reactions Involving Sperm Cells and Preimplantation Embryos
نویسندگان
چکیده
منابع مشابه
Immunologic reactions involving sperm cells and preimplantation embryos.
Evidence that sperm cells share or impart antigenic specificities to fertilized ova and cleaving embryos in animals was first demonstrated indirectly in female animals isoimmunized against sperm or testis cells. While early studies often gave conflicting results, postfertilization infertility effects were occasionally reported. The systematic analysis in animals of anti~ertility e~ects after im...
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Background This novel approach describes a rapid and simple method for identification of necrotic vs. viable cells within a mammalian blastocyst. MaterialsAndMethods Hatched bovine blastocysts produced in vitro were first incubated for 30 min in pre-equilibrated culture medium containing propidium iodide (PI; 300μg/ml) and bisbenzimide (Hoechst: H33342; 5μg/ml) fluorescent dyes. Embryos were th...
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The ability of two embryonal carcinoma (EC) cell lines, F9 and PC13, to aggregate with preimplantation 8-cell mouse embryos is described. Both adhere to the embryonic cells and subsequently compact with the embryos. The aggregates form blastocysts in culture. The blastocysts sometimes contain the EC cells, located almost always in their inner cell mass. Differentiated derivatives of EC cells, n...
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BACKGROUND Although chromosomal mosaicism in human preimplantation embryos has been described for almost two decades, its exact prevalence is still unknown. The prevalence of mosaicism is important in the context of preimplantation genetic screening in which the chromosomal status of an embryo is determined by the analysis of a single cell from that embryo. METHODS Here we report a systematic...
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Mouse two-celled embryos and blastulae were Feulgen stained and the DNA content of their nuclei was measured with an integrating microdensitometer. The cells considered on the basis of their nuclear DNA content to be in G(1), S, and G(2) phases of the cell cycle were selected and their total chromatin area and chromatin areas at different gray levels were measured by the image analyzing compute...
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ژورنال
عنوان ژورنال: American Journal of Reproductive Immunology and Microbiology
سال: 1988
ISSN: 8755-8920
DOI: 10.1111/j.1600-0897.1988.tb00227.x