Reversible catalysis

Mechanistic insights from reversible splicing catalysis.

Recent work demonstrating the ability of spliceosomes purified after the second catalytic step of splicing to efficiently reverse both steps of the reaction provides answers to several unresolved questions regarding the splicing reaction, and raises many more.

Homogeneous Catalysis Homogeneous Catalysis

A competitive and reversible deactivation approach to catalysis-based quantitative assays.

Catalysis-based signal amplification makes optical assays highly sensitive and widely useful in chemical and biochemical research. However, assays must be fine-tuned to avoid signal saturation, substrate depletion and nonlinear performance. Furthermore, once stopped, such assays cannot be restarted, limiting the dynamic range to two orders of magnitude with respect to analyte concentrations. In...

Deactivation of signal amplification by reversible exchange catalysis, progress towards in vivo application.

The catalyst which is used in the signal amplification by reversible exchange (SABRE) process facilitates substrate hyperpolarisation while acting to speed up the rate of relaxation. Consequently, the lifetime over which the hyperpolarised contrast agent is visible is drastically reduced. We show that the addition of a chelating ligand, such as bipyridine, rapidly deactivates the SABRE catalyst...

Reversible catalysis for the reaction between methyl orange and NaBH4 by silver nanoparticles.

The reaction between MO and NaBH4 catalyzed by Ag NPs has been studied. Ag NPs catalyzed the reduction of MO rapidly, while adding CTAB into the solution caused the regeneration of MO. Thus, reversible catalysis for the reaction between MO and NaBH4 by Ag NPs was discovered for the first time.