Revealing the genetic diversity of Sumbawa endemic horse using microsatellite-based DNA fingerprint

Abstract. Sukri A, Dewi IN, Primawati SB, Wangiyana IGAS, Muttaqin Z, Winaya A. 2022. Revealing the genetic diversity of Sumbawa endemic horse using microsatellite-based DNA fingerprint. Biodiversitas 23: 4153-4159. (Equus caballus) is a resource that must be preserved. horses have significant economic, social, and cultural role. Information on status needed as step in developing long-term conservation strategy. Therefore, this study aims to reveal isolation was conducted from blood samples. Blood samples were taken two farms with 24 individual different populations, namely Lalar Liang,West District, Indonesia. jugular vein collected by qualified veterinarian Faculty Veterinary Medicine, Universitas Pendidikan Mandalika. A total 4 microsatellite primers used study, INRA032, HEL09, CA425, AHT4. This revealed population higher than Liang. greater number alleles reinforces this; frequency bands; presence specific bands indicate unique alleles. research shows other breeds world. evidenced lower per locus (Na) maximum locus. observed heterozygosity (Ho) expected (He), Ho value less 0.5. Analysis Molecular Variance result has shown variation within among population. presumably due high gene flow both populations caused inbreeding. In general, AHT4 had best ability highest Shannon's information index compared markers.