Retroviral vector gene expression in F9 embryonal carcinoma cells
نویسندگان
چکیده
منابع مشابه
Increased probability of expression from modified retroviral vectors in embryonal stem cells and embryonal carcinoma cells.
Gene expression from the Moloney murine leukemia retrovirus (Mo-MuLV) is highly restricted in embryonic carcinoma (EC) and embryonic stem (ES) cells. We compared levels of expression in PA317 fibroblasts, F9 (EC) cells, and CCE (ES) cells by Mo-MuLV-based vectors and vectors based on our previously reported MND backbone, which has alterations to address three viral elements implicated as repres...
متن کاملThrombomodulin gene regulation by cAMP and retinoic acid in F9 embryonal carcinoma cells.
Thrombomodulin (TM) expression was investigated during differentiation of F9 embryonal carcinoma cells into primitive or parietal endoderm. Exposure of F9 cells to retinoic acid (RA) triggers differentiation into primitive endoderm and induces the appearance of barely detectable amounts of TM mRNA, whereas treatment with dibutyryl cAMP plus theophylline (CT) augments the levels of TM mRNA to a ...
متن کاملTargeted Deletion of #1 Integrins in F9 Embryonal Carcinoma Cells Affects Morphological Differentiation but not Tissue-specific Gene Expression
The integrin superfamily of heterodimeric transmembrane adhesion receptors mediates many cell-cell and cell-matrix interactions whose functions are believed to be critical for normal morphogenesis and differentiation. By eliminating the fll integrin gene through homologous recombination, we have assessed the role of the fll integrin family in the F9 embryonal carcinoma model for endodermal diff...
متن کاملTargeted deletion of beta 1 integrins in F9 embryonal carcinoma cells affects morphological differentiation but not tissue-specific gene expression
The integrin superfamily of heterodimeric transmembrane adhesion receptors mediates many cell-cell and cell-matrix interactions whose functions are believed to be critical for normal morphogenesis and differentiation. By eliminating the beta 1 integrin gene through homologous recombination, we have assessed the role of the beta 1 integrin family in the F9 embryonal carcinoma model for endoderma...
متن کاملevaluation of pdx-1 gene expression in insulin producing cells, derived from embryonal carcinoma stem cells
background and aims: understanding β-cell function at the molecular level will likely facilitate the development of β-cells manufacture techniques. the aim of the present study was to investigate the pancreatic and duodenal homeobox 1 (pdx-1) gene expression into dtz-stained cellular clusters originating from emberyonal carcinoma cells. methods: this implemental -fundamental study was accomplis...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1987
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.61.10.3248-3253.1987