Restriction endonuclease Ncilis not blocked by CpG methylation
نویسندگان
چکیده
منابع مشابه
The restriction enzyme AlwNI is blocked by overlapping methylation.
Most strains of E.coli contain two sequence specific methylases, Dam and Dem. The adenine residue of the sequence GATC is methylated at the N 6 position by the Dam methylase whereas the Dem methylase recognizes the sequences CC(A/T)GG methylating the internal cytosine at the C 5 position. The commercially available restriction enzyme AlwNl (New England Biolabs) has a recognition sequence CAGN 3...
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Recognizing the methylation status of specific DNA sequences is central to the function of many systems in eukaryotes and prokaryotes. Restriction-modification systems have to distinguish between 'self' and 'non-self' DNA and depend on the inability of restriction endonucleases to cleave their DNA substrates when the DNA is appropriately methylated. These endonucleases thus provide a model syst...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1993
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/21.23.5517