Restriction Endonuclease Cleavage Maps of the Ampicillin TransposonsTn2601andTn2602
نویسندگان
چکیده
منابع مشابه
Restriction endonuclease cleavage maps of rat and mouse mitochondrial DNAs.
Mitochondrial DNA from an Old World mouse, Mus musculus, and from an Old World rat, Rattus norvegicus, contain 19 and 22 distinct sites, respectively, for the 8 restriction endonucleases, BamHI, EcoRI, HaeII, HhaI, HincII, HindIII, HpaI and PstI. The relative positions of the sites have been mapped by the study of partial and double enzyme digests. Some sites may been conserverd between the mou...
متن کاملRestriction endonuclease cleavage maps of animal mitochondrial DNAs.
The restriction endonuclease, HindIII, gives rise to three fragments in each of the three mitochondrial DNAs isolated from the established mammalian cell lines LA9 (mouse), HeLa (human), and BSC-1 (African green monkey). The restriction endonuclease, EcoRI, gives rise to three fragments in mitochondrial DNA from HeLa and to two in DNAs from LA9 and BSC-1. The sizes and the orders of the fragmen...
متن کاملThe cleavage site of the restriction endonuclease Ava II.
We have determined that the type II restriction enzyme Ava II, isolated from Anabaena variabilis, recognizes and cuts the sequence (formula: see article). The eight Ava II sites of pBR322 have been mapped, as well as a unique site for Ava I.
متن کاملThe cleavage site of the restriction endonuclease Ava II
We have determined that the type II restriction enzyme . Ava II , isolated from Anabaena variabilis, recognizes and cuts the sequence 5' G-1GTCC 3' 3 CCAG+G 5' The eight Ava II sites of pBR322 have been mapped, as well as a unique site for Ava
متن کاملConstruction and validation of yeast artificial chromosome contig maps by RecA-assisted restriction endonuclease cleavage.
RecA-assisted restriction endonuclease (RARE) cleavage is an "Achilles' heel" approach to restriction mapping whereby a RecA-protein-oligodeoxynucleotide complex protects an individual restriction site from methylation, thus limiting subsequent digestion to a single, predetermined site. We have used RARE cleavage to cut yeast artificial chromosomes (YACs) at specific EcoRI sites located within ...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 1980
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.1980.tb02919.x