Restriction Endonuclease Analysis of Chlamydia trachomatis DNA
نویسندگان
چکیده
منابع مشابه
Restriction endonuclease analysis of Aspergillus fumigatus DNA.
AIMS To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould. METHODS DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA ...
متن کاملImmuno-gold Labelling of Chlamydia trachomatis
Background Chlamydia trachomatis is considered as an important cause of preventable sexually transmitted diseases worldwide. It is known to be of an obligate intracellular nature and enters its target cells via an endocytic process. As major outer membrane protein (MOMP) is one of the main candidates for the attachment and entry of chlamydia to the host cells we have tried to label the epitopes...
متن کاملComparison of avian Chlamydia psittaci isolates by restriction endonuclease analysis and serovar-specific monoclonal antibodies.
Avian Chlamydia psittaci isolates were examined by restriction endonuclease analysis and serovar-specific monoclonal antibodies and compared with ovine abortion and polyarthritis isolates. The avian isolates were divided into four serovars (turkey, psittacine, pigeon, and duck) based on their reactivity to the monoclonal antibodies. The DNA digest patterns were similar across the four avian ser...
متن کاملChlamydia trachomatis.
The frequency of positive serological tests for Chlamydia trachomatis, the most common sexually transmitted pathogen in the United States, was assessed in 18,622 specimens derived from New Jersey clinics. From the results, 13 percent female patients and 17 percent male patients were reactive for chlamydial antigen.
متن کامل[Chlamydia trachomatis].
The sensitivity and specificity of a newly developed ligase chain reaction (LCR) test kit were examined by the use of highly purified elementary bodies (EBs) and in situ inclusions containing reticulate bodies only. The performance of the LCR kit was compared with a commercially available polymerase chain reaction (PCR) test kit, AMPLICOR Chlamydia trachomatis. The number of EBs and inclusions ...
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ژورنال
عنوان ژورنال: Journal of the Japanese Association for Infectious Diseases
سال: 1991
ISSN: 1884-569X,0387-5911
DOI: 10.11150/kansenshogakuzasshi1970.65.730