Resistance of cloned cytotoxic T lymphocytes to cell-mediated cytotoxicity.

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Resistance of cloned cytotoxic T lymphocytes to cell-mediated cytotoxicity

Cloned CTLs show an unusually high resistance to lysis by effector CTLs. Several cloned CTL lines in our laboratories are absolutely refractory to lysis by other cloned CTLs, either (a) directly, (b) in the presence of lectin, or (c) by PMA-induced CTLs. They can be lysed to some extent by primary CTL, although they are less than 5% as sensitive as target cells normally used to assay primary CT...

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Recent evidence has shown that cloned, murine CTL cell lines are resistant to the cytotoxic components of the toxic granules they release upon specific interaction with their target cells. Inasmuch as the resistance might be due to selection in culture over many months by repeated exposure to these cytolytic components (which are released repeatedly as a result of the cultured CTL being periodi...

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Antigen recognition by cytotoxic lymphocytes (CTL) 1 and by helper T cells is restricted by gene products of the major histocompatibility complex (MHC), called H-2 in the mouse (1). CTL recognize antigens in the context of Kand D-regionencoded structures, whereas helper T cells recognize antigen in the context of I-regionencoded structures. This H-2 restriction appears to be very tight: C T L s...

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Resistance of cytotoxic T lymphocytes to lysis by a clone of cytotoxic T lymphocytes.

To investigate how cytotoxic T lymphocytes (CTL) avoid killing themselves when they destroy target cells, we compared 20 different cell lines as target cells, including several CTL cell lines, for their susceptibility to lysis by CTL. Variations in recognition of this diverse set of target cells was circumvented by attaching to all of them a monoclonal antibody to the antigen-specific receptor ...

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ژورنال

عنوان ژورنال: Journal of Experimental Medicine

سال: 1987

ISSN: 0022-1007,1540-9538

DOI: 10.1084/jem.166.4.1070