Reporter constructs with low background activity utilizing the cat gene

Reporter constructs with low background activity utilizing the ca t gene ( Recombinant

Reporter genes allowing functional analysis of eukaryotic promoters and regulatory cis-elements by a simple enzymatic assay (CAT, luciferase,/~-galactosidase, etc.) are widely used. A major caveat of these assays is that expression of enzymatic activity may result from transcription initiation within the vector. Also, vector sequences may affect the activity of the promoter under test. The form...

Assessment of immunity to mycobacterial infection with luciferase reporter constructs.

Protective immunity to mycobacterial infection is incompletely understood but probably involves the coordinated interaction of multiple cell types and cytokines. With the aim of developing assays that might provide a surrogate measure of protective immunity, we have investigated the use of recombinant mycobacteria carrying luciferase reporter enzymes to assess the effectiveness of antimycobacte...

Comparison of ELISA and TLC for the analysis of promoter activity when using the CAT reporter gene.

Transcriptional modulation of viral reporter gene constructs following induction of the cellular stress response.

In this study, we report that commonly used methods of transient transfection induce the cellular stress response and a recovery period is required following transfection when analyzing cellular stress responsive genes. Four transfection methods were examined for their ability to induce the stress response by measuring the expression of heat shock protein (hsp) 72. We demonstrate that electropo...

Employing BAC-reporter constructs in the sea anemone Nematostella vectensis.

Changes in the expression and function of genes drive evolutionary change. Comparing how genes are regulated in different species is therefore becoming an important part of evo-devo studies. A key tool for investigating the regulation of genes is represented by bacterial artificial chromosomes (BAC)-reporter constructs. BACs are large insert libraries, often >100 kb, which thus capture the geno...