Repair of Alkylation Damage: Stability of Methyl Groups in Bacillus subtilis Treated with Methyl Methanesulfonate
نویسندگان
چکیده
منابع مشابه
Methyl Methanesulfonate
Bacillus subtilis was not inactivated and was able to replicate even though approximately 3 X 104 methyl groups added by methyl methanesulfonate (MMS) were bound to the deoxyribonucleic acid (DNA) of each organism. No significant loss of methyl groups from the DNA occurred for several generations upon incubation of methylated wild-type or MMS-sensitive cells. Single-strand breaks were not obser...
متن کاملHydroalcoholic and polyphenolic extracts of Ziziphus Jujuba Mill fruits prevent methyl methanesulfonate-induced DNA damage in HepG2 cells
Ziziphus jujuba Mill (ZJ), which has been extensively used by the Iranian traditional healers, belongs to the Ramnaceae family. This semitropical herb contains large quantities of polyphenols and flavonoids, which in turn reveal antioxidant, antibacterial, free radical scavengering, and several other pharmacological activities. The purpose of the present study was to evaluate the DNA damage pre...
متن کاملNonuniform distribution of DNA repair in chromatin after treatment with methyl methanesulfonate.
The distribution of methyl methanesulfonate induced DNA repair was measured in mouse mammary cell chromatin by digestion of "repair labeled" nuclei with micrococcal nuclease. The results indicate that there is a nonuniform distribution of DNA repair in chromatin. The chromatin fraction digested during the first 5 minutes of incubation with micrococcal nuclease appears to be a primary site of DN...
متن کاملAccumulation of an intermediate in DNA synthesis by HEp.2 cells treated with methyl methanesulfonate.
A portion of the DNA synthesized by HEp.2 cells after short incubation in the presence of BrdU bands in a CsCl gradient at a distinct peak of density 1.715-1.718 g/cm(3). Inhibition of DNA synthesis by methyl methanesulfonate results in an increased proportion of this "intermediate density" DNA. Cells labeled and subsequently chased in nonradioactive BrdU yield only hybrid DNA. Treatment with s...
متن کاملMETHYL METHANESULFONATE MUTAGENESIS IN BACTERIOPHAGE T4l
MMS induces diverse rII mutations from a wild-type background in bacteriophage T4. About 56% are base pair substitutions, about 30% are frameshift mutations, and the remainder is a miscellaneous set of rapidly reverting or leaky mutants of unknown composition; but deletions were not detected. MMSinduced forward mutation is sharply reduced by the mutations px and y, which also reduce ultraviolet...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1970
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.102.3.760-766.1970