Renaturation of Spinach Leaf Glyoxylic Acid Reductase
نویسندگان
چکیده
منابع مشابه
Sulfhydryl studies of spinach leaf glyoxylic acid reductase.
Spinach leaf glyoxylic acid reductase, an enzyme containing two identical or similar subunits, has been shown to have 12 half-cystine residues. Four of these are necessary for catalytic function and can be titrated as free sulfhydryl groups. One mole of cysteinyl residues per mole of enzyme is essential to catalytic activity when evaluated under conditions favoring optimal activity. All free su...
متن کاملThe kinetic properties of spinach leaf glyoxylic acid reductase.
The kinetic properties of spinach leaf glyoxylic acid reductase have been evaluated. In the presence of pyridine nucleotides, the enzyme catalyzes the reversible reduction of glyoxylate to glycolate and hydroxypyruvate to D( -)glycerate. The pH optima of the reductive reactions are between 6.0 and 6.5 ; the pH optima of the oxidative reactions are at 8.9. The enzyme is competitively inhibited b...
متن کاملOxidation and reduction of glycolic and glyoxylic acids in plants. II. Glyoxylic acid reductase.
Clagett, Tolbert, and Burris (1) discovered an enzyme of wide-spread occurrence in the green parts of many plants which catalyzes the oxidation of glycolic and L-lactic acids. The enzyme, which was partially purified from tobacco leaves, seemed to catalyze the oxidation of glycolic acid, through glyoxylic acid, to formic acid and CO, (2). In view of the possible importance of glycolic acid oxid...
متن کاملMetabolic Interactions between Spinach Leaf Nitrite Reductase and Ferredoxin-NADP Reductase: Competition for Reduced Ferredoxin.
Steady state rates of NADP reduction decline upon commencement of nitrite reduction in reconstituted chloroplast preparations. Similarly, steady state rates of nitrite reduction are lower, but not zero, during concurrent NADP reduction. These results imply that competition for substrate occurs and suggest that nitrite reduction can successfully compete for reduced ferredoxin, even at high rates...
متن کاملGlyoxylic acid oxidation by rat liver.
The enzymatic formation of glyoxylate from tricarboxylic acids has focused attention on this cu-keto acid as a possible intermediate of alternate metabolic pathways (l-3). Although the isocitritase reaction has not been shown in animal tissues, recent work has indicated that the principal degradative pathway for glycine by rat liver preparations proceeds via glyoxylic acid (4). Although the oxi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62928-x