Renaturation of denatured, covalently closed circular DNA.
نویسندگان
چکیده
منابع مشابه
Detection of HBV Covalently Closed Circular DNA
Chronic hepatitis B virus (HBV) infection affects approximately 240 million people worldwide and remains a serious public health concern because its complete cure is impossible with current treatments. Covalently closed circular DNA (cccDNA) in the nucleus of infected cells cannot be eliminated by present therapeutics and may result in persistence and relapse. Drug development targeting cccDNA ...
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Primary duck hepatocytes were infected with a mutant duck hepatitis B virus defective in envelope protein but competent for viral DNA synthesis. Cells infected by this mutant accumulated higher levels of viral covalently closed, circular DNA (cccDNA) than those infected by wild-type virus. The accumulation of high levels of cccDNA was due to a failure of the mutant-infected cells to suppress de...
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Hepadnavirus covalently closed circular (ccc) DNA is the bona fide viral transcription template, which plays a pivotal role in viral infection and persistence. Upon infection, the non-replicative cccDNA is converted from the incoming and de novo synthesized viral genomic relaxed circular (rc) DNA, presumably through employment of the host cell's DNA repair mechanisms in the nucleus. The convers...
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The alkaline pH range over which the double stranded circular DNA of the replicative form of the bacterial virus, 4x174, is reversibly denatured has been determined in order to specify accurately the conditions for a preparative procedure for this DNA based on selective alkali denaturation. The denaturation remains entirely reversible to pH 12.6 at an ionic strength of about 0.25 over a range i...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1981
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)43352-2