Relictual tRNAs Recognized not Chemically Inert Amino Acids, but Chemically Active Aminoacyl-Adenylates
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چکیده
منابع مشابه
Rapid selection of aminoacyl-tRNAs based on biotinylation of α-NH2 group of charged amino acids
A rapid selection procedure to separate low amounts of aminoacylated tRNAs from large pools of inactive variants is described. The procedure involves a threestep protocol. After initial aminoacylation of a tRNA pool, N-hydroxysuccinimide ester chemistry is applied to biotinylate the α-NH2 group of the amino acid bound to the 3′-end of a tRNA. The biotin tag is used to capture the derivatized tR...
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Albanese, A. A. & Frankston, J. E. (1945). John8 Hopk. Ho8p. Bull. 77, 61. Astwood, E. B., Bissell, A. & Hughes, A. M. (1945). Endocrinology, 37, 456. Bergmann, M. ,& Zervas, L. (1928). Biochem. Z. 203, 280. Burroughs, E. W., Burroughs, H. S. & Mitchell, H. H. (1940). J. Nutrit. 19, 363. Conrad, R. M. & Berg, C. P. (1936). J. biol. Chem. 117, 351. Cox, G. J. & Rose, W. L. (1926). J. biol. Chem....
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ژورنال
عنوان ژورنال: Ecology and Evolutionary Biology
سال: 2017
ISSN: 2575-3789
DOI: 10.11648/j.eeb.20170203.12