Regulation of microtubule assembly by human EB1 family proteins
نویسندگان
چکیده
منابع مشابه
Eb1 Proteins Regulate Microtubule Dynamics, Cell Polarity, and Chromosome Stability
The EB1 family represents a highly conserved group of proteins, present in yeast through humans, that localize to spindle and cytoplasmic microtubules, especially at their distal tips. The budding yeast homologue of EB1, Bim1p, regulates microtubule stability and is important for positioning the mitotic spindle, anchoring it to the bud through astral microtubule attachment to the cortical prote...
متن کاملSpectraplakins promote microtubule-mediated axonal growth by functioning as structural microtubule-associated proteins and EB1-dependent +TIPs (tip interacting proteins).
The correct outgrowth of axons is essential for the development and regeneration of nervous systems. Axon growth is primarily driven by microtubules. Key regulators of microtubules in this context are the spectraplakins, a family of evolutionarily conserved actin-microtubule linkers. Loss of function of the mouse spectraplakin ACF7 or of its close Drosophila homolog Short stop/Shot similarly ca...
متن کاملThe microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization.
Several microtubule-binding proteins including EB1, dynactin, APC, and CLIP-170 localize to the plus-ends of growing microtubules. Although these proteins can bind to microtubules independently, evidence for interactions among them has led to the hypothesis of a plus-end complex. Here we clarify the interaction between EB1 and dynactin and show that EB1 binds directly to the N-terminus of the p...
متن کاملRegulation of microtubule assembly in cultured fibroblasts
Microtubule assembly in diploid human skin fibroblasts was studied by [3H]colchicine binding to disaggregated microtubule subunits and to total cell tubulin. Microtubule content per milligram of cell protein was critically dependent upon cell density. As cultures neared confluence, microtubules increased and total cell tubulin decreased; the percent of tubulin assembled into microtubules increa...
متن کاملMicrotubule plus-end tracking by CLIP-170 requires EB1.
Microtubules are polarized polymers that exhibit dynamic instability, with alternating phases of elongation and shortening, particularly at the more dynamic plus-end. Microtubule plus-end tracking proteins (+TIPs) localize to and track with growing microtubule plus-ends in the cell. +TIPs regulate microtubule dynamics and mediate interactions with other cellular components. The molecular mechan...
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ژورنال
عنوان ژورنال: Oncogene
سال: 2001
ISSN: 0950-9232,1476-5594
DOI: 10.1038/sj.onc.1204429