Reduction of dual‐species biofilm after sonic‐ or ultrasonic‐activated irrigation protocols: A laboratory study

Aim To evaluate the antibacterial effect of sonic- and ultrasonic-activated irrigation on bacterial reduction a dual-species biofilm in root canals compared to nonactivated laboratory study. Methodology Two hundred forty extracted human single-rooted maxillary anterior teeth were divided into two main groups (G, n = 120) according initial preparation size canal (G1: 25, 0.06 taper, G2: 40, taper). Root inoculated with Enterococcus faecalis Streptococcus oralis. After 5 days, G1 received combined instrumentation (up taper) irrigation/activation, whereas G2 solely irrigation/activation protocols. In both groups, was performed sodium hypochlorite (NaOCl 1%) or physiological saline (NaCl 0.9%), using syringe irrigation, sonic activation (2 x 30 s) ultrasonic s). Logarithmic factors (LRFs) colony-forming units analysed separately for dentine-adherent planktonic bacteria immediately after protocols (time-point 1) days further incubation 2) by analysis variance (anova) post hoc tests (Tukey's HSD, t-test). The significance level set at 0.05. Results subgroups (combined irrigation/activation), LRFs significantly affected applied solution (p < .0001), but not method > .05; anova). (solely both, irrigant activation, .0001, Sonic resulted higher than .0001) which had greater reductions Tukey's HSD). At T2, strong regrowth observed all groups; however, significant detected instrumentation, .0001; Similar found cells (r 0.91 T1, r 0.8 T2). Conclusions this study high-frequency receiving protocols; NaOCl also contributed control groups.