Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells
نویسندگان
چکیده
منابع مشابه
Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells
We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5- FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1...
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Colon cancer is a leading cause of cancer-related death in developed countries. Although 5-fluorouracil (5FU) has traditionally been studied for its potential to induce apoptosis, its clinical use had been greatly limited as a result of the development of drug resistance in patients. Besides the medicines that could induce apoptosis in cancer cells, several other alternative treatments, which h...
متن کاملMicroRNA Profiling in Human Colon Cancer Cells during 5-Fluorouracil-Induced Autophagy
Autophagy modulation is now recognized as a potential therapeutic approach for cancer (including colorectal cancer), yet the molecular mechanisms regulating autophagy in response to cellular stress are still not well understood. MicroRNAs (miRNAs) have been found to play important roles in controlling many cellular functions, including growth, metabolism and stress response. The physiological i...
متن کاملDecreased expression of hMLH1 correlates with reduced 5-fluorouracil-mediated apoptosis in colon cancer cells.
Patients with sporadic microsatellite instable colorectal cancers, in most of which the function of the hMLH1 mismatch repair gene is impaired, do not gain a survival benefit from 5-fluorouracil (5-FU)-based chemotherapy. However, the effect of hMLH1 on the cytotoxicity induced by 5-FU has not yet been sufficiently confirmed. In this study, we assessed the effect of hMLH1 on cytotoxicity and ap...
متن کاملInteraction of DNA demethylase and histone methyltransferase upregulates Nrf2 in 5-fluorouracil-resistant colon cancer cells
We recently reported that DNA demethylase ten-eleven translocation 1 (TET1) upregulates nuclear factor erythroid 2-related factor 2 (Nrf2) in 5-fluorouracil-resistant colon cancer cells (SNUC5/5-FUR). In the present study, we examined the effect of histone modifications on Nrf2 transcriptional activation. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) were respectively decreased...
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ژورنال
عنوان ژورنال: Biomolecules & Therapeutics
سال: 2017
ISSN: 2005-4483,1976-9148
DOI: 10.4062/biomolther.2016.069