Reconstitution photoactive yellow protein from apoprotein and p -coumaric acid derivatives
نویسندگان
چکیده
منابع مشابه
Controlling electron emission from the photoactive yellow protein chromophore by substitution at the coumaric acid group.
Understanding how the interactions between a chromophore and its surrounding protein control the function of a photoactive protein remains a challenge. Here, we present the results of photoelectron spectroscopy measurements and quantum chemistry calculations aimed at investigating how substitution at the coumaryl tail of the photoactive yellow protein chromophore controls competing relaxation p...
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Controlling the Photoreactivity of the Photoactive Yellow Protein Chromophore by Substituting at the p-Coumaric Acid Group Martial Boggio-Pasqua and Gerrit Groenhof* Laboratoire de Chimie et Physique Quantiques, IRSAMC, CNRS et Universit e de Toulouse, 31062 Toulouse, France Computational Biomolecular Chemistry group, Max-Planck-Institut f€ur Biophysikalische Chemie, Am Fassberg 11, D-37077 G€o...
متن کاملControlling the Photoreactivity of the Photoactive Yellow Protein Chromophore by Substituting at the p-Coumaric Acid Group
We have performed ab initio CASSCF, CASPT2, and EOM-CCSD calculations on doubly deprotonated p-coumaric acid (pCA(2-)), the chromophore precursor of the photoactive yellow protein. The results of the calculations demonstrate that pCA(2-) can undergo only photoisomerization of the double bond. In contrast, the chromophore derivative with the acid replaced by a ketone (p-hydroxybenzylidene aceton...
متن کاملChemical reactivity and spectroscopy of the thiol ester-linked p-coumaric acid chromophore in the photoactive yellow protein from Ectothiorhodospira halophila.
We have recently identified p-coumaric acid as the chromophore of the photoactive yellow protein (PYP) from the purple sulfur bacterium Ectothiorhodospira halophila, a blue-light photoreceptor with rhodopsin-like photochemistry [Hoff, W. D., Düx, P., Hård, K., Nugteren-Roodzant, I. M., Crielaard, W., Boelens, R., Kaptein, R., Van Beeumen, J., & Hellingwerf, K. J. (1994) Biochemistry 33, 13959-1...
متن کاملUnraveling the similarity of the photoabsorption of deprotonated p-coumaric acid in the gas phase and within the photoactive yellow protein.
Using advanced QM/MM methods, the surprisingly negligible shift of the lowest-lying bright electronic excitation of the deprotonated p-coumaric acid (pCA(-)) within the photoactive yellow protein (PYP) is shown to stem from a subtle balance between hypsochromic and bathochromic effects. More specifically, it is found that the change in the excitation energy as a consequence of the disruption of...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1995
ISSN: 0014-5793
DOI: 10.1016/0014-5793(95)01096-w