Reconstitution of the Na+/H+ antiporter: A new method for the determination of H+ efflux from Na+/H+ antiporter-reconstituted vesicles.
نویسندگان
چکیده
منابع مشابه
Functional expression of the Enterococcus hirae NaH-antiporter in Escherichia coli.
We recently described the cloning of napA, the putative structural gene for the NaH-antiporter of Enterococcus hirae (Waser, M., Bienz-Hess, D., Davies, K., and Solioz, M. (1992) J. Biol. Chem. 267, 5396-5400). To analyze the gene product of napA, we expressed it in Escherichia coli. When placed under the control of a T7 promoter, napA could be transcribed and labeled specifically with [35S]met...
متن کاملCloning and disruption of a putative NaH-antiporter gene of Enterococcus hirae.
When growing in a sodium-rich environment, wild-type Enterococcus hirae extrudes sodium by two mechanisms, ATP-driven sodium extrusion, and NaH-antiport. Mutant 7683 is unable to grow on sodium-rich media. This is due to two mutations, one inactivating ATP-driven sodium transport and a second rendering NaH-antiport inoperative. 7683 was transformed by electroporation with a gene bank, derived f...
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The leucine zipper, EF hand-containing transmembrane protein 1 (Letm1) gene encodes a mitochondrial inner membrane protein, whose depletion severely perturbs mitochondrial Ca(2+) and K(+) homeostasis. Here we expressed, purified, and reconstituted human Letm1 protein in liposomes. Using Ca(2+) fluorophore and (45)Ca(2+)-based assays, we demonstrate directly that Letm1 is a Ca(2+) transporter, w...
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ژورنال
عنوان ژورنال: Journal of Pharmacobio-Dynamics
سال: 1988
ISSN: 0386-846X,1881-1353
DOI: 10.1248/bpb1978.11.669