Reconstitution of Recombinant Uncoupling Proteins
نویسندگان
چکیده
منابع مشابه
A new automated technique for the reconstitution of hydrophobic proteins into planar bilayer membranes. Studies of human recombinant uncoupling protein 1.
Electrophysiological characterisation of the vast number of annotated channel and transport proteins in the postgenomic era would be greatly facilitated by the introduction of rapid and robust methods for the functional incorporation of membrane proteins into defined lipid bilayers. Here, we describe an automated technique for reconstitution of membrane proteins into lipid bilayer membranes, wh...
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The aim of this review is to provide an overview of current knowledge on the three mammalian uncoupling proteins, UCP-1, UCP-2, and UCP-3. Heart Metab. 2010;48:32–34.
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Uridine (U)-insertion/deletion RNA editing in trypanosome mitochondria involves an initial cleavage of the preedited mRNA at specific sites determined by the annealing of partially complementary guide RNAs. An involvement of two RNase III-containing core editing complex (L-complex) proteins, MP90 (KREPB1) and MP61 (KREPB3) in, respectively, U-deletion and U-insertion editing, has been suggested...
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The uncoupling protein-1 (UCP1), cloned in 1985 (1– 4) and called UCP until 1997, is an inner mitochondrial membrane protein (5) expressed exclusively in the brown adipocyte (6–8). It dissipates the mitochondrial proton (H) gradient generated by the respiratory chain, producing heat instead of ATP (8, 9). In rodents the brown adipose tissue (BAT) contributes to both the maintenance of body temp...
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Uncoupling proteins (UCPs) are mitochondrial transporters present in the inner membrane of mitochondria. They are found in all mammals and in plants. They belong to the family of anion mitochondrial carriers including adenine nucleotide transporters. The term "uncoupling protein" was originally used for UCP1, which is uniquely present in mitochondria of brown adipocytes, the thermogenic cells t...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2003
ISSN: 0021-9258
DOI: 10.1074/jbc.m302126200