Recommendations for conducting the rodent erythrocyte <i>Pig?a</i> assay: A report from the <scp>HESI GTTC</scp> <i>Pig?a</i> Workgroup

The rodent Pig-a assay is a flow cytometric, phenotype-based method used to measure in vivo somatic cell mutation. An Organization for Economic Co-operation and Development (OECD) test guideline currently being developed support routine use of the regulatory purposes (OECD project number 4.93). This article provides advice on best practices designing conducting studies evaluating substance safety, with focus rat model. Various aspects conduct, including laboratory proficiency, minimum animals per dose group, preferred treatment blood sampling schedule, statistical analysis are described.