Recombination‐Based Assay ( RBA ) for Screening Bacteriophage Lambda Libraries

Deep screening of recombination proficient bacteriophage libraries.

We are using bacteriophage λ as an efficient vector for transferring genomic alterations to embryonic stem (ES) cells via gene targeting. We have developed a variety of phage-plasmid recombination techniques to direct mutations and modification cassettes to specific sites within phage targeting vectors (1,6–9). We have also made improvements on Seed’s original method of recombination screening ...

Improved Procedure for Screening Expression Libraries for Novel Autoantigens

The standard method for immunoscreening of a cDNA expression library is time-consuming becauseof the production of a large proportion of false positives during the first and second round of screening.This problem is more important when a sensitive chemiluminescence detection system is used. Due tothe high sensitivity of the detection system, there is a need to avoid false posi...

Genetic map of bacteriophage lambda.

Genetic recombination in bacteriophage lambda

© The Author 2012. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Genetic recombination in bacteriop...

Novel bacteriophage lambda mutation affecting lambda head assembly.

A novel phage lambda mutation, called dc10, which interferes with proper lambda head assembly has been isolated and characterized. Phage lambda carrying this mutation is (i) unable to form plaques at 30 or 37 degrees C but does so at 42 degrees C and (ii) unable to form plaques at 42 degrees C on pN-constitutive hosts. Both properties are due to dc10 since all phage revertants for one phenotype...