Recombinant production, purification and crystallization of theToxoplasma gondiicoronin WD40 domain
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چکیده
منابع مشابه
Bacterially expressed recombinant WD40 domain of human Apaf-1.
The apoptotic protease activating factor (Apaf-1) is a protein that binds to cytochrome c, and in the presence of dATP/ATP oligomerizes to assume the role of an adaptor platform for activating the caspase-9 zymogen. In order to study the biochemical and structural details of Apaf-1 function, we have generated an expression construct from pcDNA 3-Apaf-1XL for production of the WD40 domain ((WD40...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology Communications
سال: 2014
ISSN: 2053-230X
DOI: 10.1107/s2053230x14005196