Recombinant Lamb Chymosin as an Alternative Coagulating Enzyme in Cheese Production
نویسندگان
چکیده
منابع مشابه
Production of Bioactive Recombinant Bovine Chymosin in Tobacco Plants
Chymosin (also known as rennin) plays an essential role in the coagulation of milk in the cheese industry. Chymosin is traditionally extracted from the rumen of calves and is of high cost. Here, we present an alternative method to producing bovine chymosin from transgenic tobacco plants. The CYM gene, which encodes a preprochymosin from bovine, was introduced into the tobacco nuclear genome und...
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Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain could serve as a novel source for rennet production. Yaks total RNA was extracted from the abomasum of an unweaned yak. The yak preprochymosin, prochymosin, and chymosin genes from total RNA were is...
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متن کامل[Recombinant expression of bovine chymosin in Pichia pastoris].
To express bovine chymosin in yeast, we amplified the prochymosin gene from the plasmid pMD18T-Prochy by PCR, and then cloned the gene into the expression vector pPICZaA, resulting in pPICZaA-Prochy. Pichia pastoris GS115 was used as host cells. Integration of the prochymosin cDNA into the Pichia pastoris genome was confirmed by PCR and sequencing analysis. Chymosin was expressed in Pichia past...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 2001
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(01)74561-4