Recognition of bovine hemoglobin protein on molecularly imprinted polymer surfaces using nonlinear vibrational spectroscopy

Advancement in molecularly imprinted biomimetics has aided developing robust artificial recognition-based materials, which can be customized for bio/chemo-sensing of distinct molecules. The present study reports a simple one-step synthesis and analysis protein-imprinted polymer thin films as recognition element directly onto solid support. Dopamine been explored versatile functional monomer (MIP) matrix to fabricate polydopamine (PDA) with bovine hemoglobin template protein molecule. A detailed molecular-level insight into the molecule at each step investigated using vibrational sum frequency generation (VSFG) spectroscopy. suitable PDA-coated film is selected based on extent polymerized intermediates formed after non-imprinted fabrication different time durations. An optimally prepared MIP specified by observing spectral signature methyl groups from molecules air–polymer interface. removal washing procedure subsequent re-binding were evaluated VSFG insightful findings SFG spectroscopy demonstrate an label-free chemical sensor.