Real-Time RT-PCR on SAG1 and BAG1 Gene Expression during Stage Conversion in Immunosuppressed Mice Infected with Toxoplasma gondii Tehran Strain

Real-Time RT-PCR on SAG1 and BAG1 Gene Expression during Stage Conversion in Immunosuppressed Mice Infected with Toxoplasma gondii Tehran Strain

Toxoplasmic encephalitis is caused by reactivation of bradyzoites to rapidly dividing tachyzoites of the apicomplexan parasite Toxoplasma gondii in immunocompromised hosts. Diagnosis of this life-threatening disease is problematic, because it is difficult to discriminate between these 2 stages. Toxoplasma PCR assays using gDNA as a template have been unable to discriminate between an increase o...

Brain Tissue Cysts in Infected Mice with RH-Strain of Toxoplasma gondii and Evaluation of BAG1 and SAG1 Genes Expression

BACKGROUND Toxoplasma gondii is an obligate intracellular parasite that infects humans at high prevalence rates. The virulent RH strain of T. gondii is generally considered to have lost its cyst forming capacity. This study performed to obtain tissue cysts in mice infected with tachyzoites of RH strain treated with sulfadiazine (SDZ). It provides the opportunity to analyze the conversion of tac...

brain tissue cysts in infected mice with rh-strain of toxoplasma gondii and evaluation of bag1 and sag1 genes expression.

background : toxoplasma gondii is an obligate intracellular parasite that infects humans at high preva-lence rates. the virulent rh strain of t. gondii is generally considered to have lost its cyst forming ca-pacity. this study performed to obtain tissue cysts in mice infected with tachyzoites of rh strain treated with sulfadiazine (sdz). it provides the opportunity to analyze the conversion of...

Anterior uveitis in a kitten infected with Toxoplasma gondii (Tehran strain)

Ocular lesions are a common manifestation of toxoplasmosis in cats. In this survey, 10 healthy Syrianmice were infected through intraperitoneal inoculation of bradyzoites of Toxoplasma gondii (Tehran strain).Mice were killed 30 days after inoculation and the suspension of their brains was prepared, after confirmationof the infection. Seven kittens were fed with the infected mice brain suspensio...

Toxoplasma Gondii Real Time PCR Kit