Real-Time Quantitative-PCR (qrt-PCR) Using Alleleseqr Technology: to Assess Chimerism in Pediatrics Patients Undergoing Hematopoietic Stem Cell Transplantation (HSCT)

Digital PCR to assess hematopoietic chimerism after allogeneic stem cell transplantation.

Analysis of hematopoietic chimerism after allogeneic stem cell transplantation represents a crucial method to evaluate donor-cell engraftment. Whereas sensitivity of classical approaches for chimerism monitoring is limited to ≥1%, quantitative polymerase chain reaction (qPCR)-based techniques readily detect one patient cell in >1,000 donor cells, thus facilitating application of chimerism asses...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Chimerism analysis in sex-mismatched murine transplantation using quantitative real-time PCR.

Marine experimental stem cell transplantations require the accurate discrimination and quantification of donor cells from host cells. A Y-chromosome-specific, quantitative real-time PCR (kinetic PCR) protocol for blood-derived DNA was developed. The assay sensitivity was extremely high with accurate detection of only 10 pg (six copies of Y target DNA) in a variable background of female DNA back...

Oral Ciprofloxacin Prophylaxis in Patients Undergoing High Dose Therapy and Autologous Hematopoietic Stem Cell Transplantation

Background: Antibiotic prophylaxis is usually used in allogenic stem cell transplantation, but its use in Autologous Stem Cell Transplantation (ASCT) is controversial. We evaluated the efficacy of ciprofloxacin prophylaxis in ASCT. Materials and Methods: To identify the efficacy of ciprofloxacin on the incidence of neutropenic fever and its complications, 72 patients had been admitted to Talegh...

the relationship between str-pcr chimerism analysis and chronic gvhd following hematopoietic stem cell transplantation

background: the study attempts to assess the relationship between chimerism analysis using polymerase chain reaction of short tandem repeat (str) and the incidence of chronic graft versus host disease (gvhd) as well as survival. methods: the retrospective cohort included all patients who received allo-hsct between 2005 to 2013. data collected by day +100 were reviewed in terms of the incidence ...