Real-time quantitative PCR array to study drug-induced changes of gene expression in tumor cell lines
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چکیده
منابع مشابه
designing of relative quantitative real-time pcr for detection of cd40 gene expression in dendritic cell after suppression with antisense
objective: dendritic cells have a critical role in control and regulation of immune responses. it is believed that these cells can be used for the treatment of many diseases. one of the methods used in immunotherapy is based on generating of tolerogenic dendritic cells through inhibition of expression costimulatory molecules. cd40 is one of the costimulatory molecules, and inhibition of express...
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Today, quantitative real-time PCR is the method of choice for rapid and reliable quantification of mRNA transcription. However, for an exact comparison of mRNA transcription in different samples or tissues it is crucial to choose the appropriate reference gene. Recently glyceraldehyde 3-phosphate dehydrogenase and beta-actin have been used for that purpose. However, it has been reported that th...
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ژورنال
عنوان ژورنال: Journal of Cancer Metastasis and Treatment
سال: 2017
ISSN: 2454-2857,2394-4722
DOI: 10.20517/2394-4722.2017.22